BEGIN:VCALENDAR
VERSION:2.0
PRODID:icalendar-ruby
CALSCALE:GREGORIAN
METHOD:PUBLISH
BEGIN:VEVENT
DTSTAMP:20261005T122029Z
UID:https://www.mr.mpg.de/events/14864/10694
DTSTART:20190109T140000Z
DTEND:20190109T150000Z
CLASS:PUBLIC
CREATED:20180731T124003Z
DESCRIPTION:<i>Normal and Attenuated Diffusion in the Cell and its effect o
 n protein Activity:</i> Biochemistry traditionally determines protein-acti
 vity in dilute solutions\, while their native environment is the densely p
 opulated cytoplasm of the living cell. The environment of the cytoplasm is
  very different from the test tube in its composition and crowding. This r
 esults in potential hard and soft interactions\, which may affect diffusio
 n and binding. In my talk I will provide experimental evidence on how thes
 e interactions affect catalytic activity and protein-protein interactions.
  For catalytic activity we found that in vivo catalytic efficiency varied 
 between cells and was much lower than in vitro. Simulations and experiment
 s showed that the attenuated diffusion of the substrate related to the red
 uced apparent enzyme activity in the cell. For protein-protein interaction
 s we followed both structured and natively unfolded proteins in the cell. 
 Here\, we found the cellular environment to have a more limited effect on 
 binding. We suggest that this is a result of proteins having evolved to ba
 lance between binding desired partners while rejecting others\, achieving 
 fast and specific interactions. Indeed\, we show that very few mutations a
 re required to evolve new bind partners\, which may effect homeostasis in 
 the cell.
LAST-MODIFIED:20190108T093600Z
LOCATION:MPI for Medical Research\, Room: Seminar Room A/B
ORGANIZER;CN=MPI for Medical Research:mailto:
SUMMARY:Rudolf Mößbauer Colloquium with Gideon Schreiber (Weizmann Instit
 ute of Science): Rudolf Mößbauer Colloquium with Gideon Schreiber (Weizm
 ann Institute of Science)
URL;VALUE=URI:https://www.mr.mpg.de/events/14864/10694
END:VEVENT
END:VCALENDAR