BEGIN:VCALENDAR VERSION:2.0 PRODID:icalendar-ruby CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT DTSTAMP:20260614T041632Z UID:https://www.mr.mpg.de/events/19259/10694 DTSTART:20191128T140000Z DTEND:20191128T153000Z CLASS:PUBLIC CREATED:20190705T104822Z DESCRIPTION:During mitotic cell division\, each daughter cell receives from its mother cell an exact\, full copy of the genome. For this to happen\, the sister chromatids in the mother cell must bi-orient on the mitotic spi ndle. Sister chromatid separation at the metaphase-to-anaphase transition then leads to equal segregation of the genome to the two daughters. Chromo some attachment to spindle microtubules takes place at complex protein str uctures named kinetochores\, which contain multiple copies of as many as ~ 30 individual core subunits. This stable protein core emerges from a speci alized region of the chromosome known as the centromere. Microtubule bindi ng by kinetochores is subject to a feedback control mechanism known as err or correction (ER)\, and whose purpose is to detect improper configuration s of the attachments and allow their regression. This mechanism is believe d to require a force sensor capable of monitoring differences in the actio n of forces acting on kinetochores when they are bi-oriented (correct atta chments) or not (incorrect attachment). The molecular nature of this force sensor remains unclear. In addition\, kinetochores determine the timing o f mitotic exit by exercising control over the cell cycle machinery through the spindle assembly checkpoint (SAC). The SAC coordinates completion of bi-orientation with the transition to anaphase\, preventing premature mito tic exit in the presence of incompletely attached sister chromatid pairs. All SAC components are recruited to kinetochores and regulated there in a way that reflects attachment status but that remains poorly understood. In the last several years\, our laboratory engaged in the in vitro re constitution and in the structural and functional characterization of seve ral kinetochore sub-complexes that operate at the interface between chroma tin and microtubules. We also reconstituted crucial aspects of SAC signall ing\, identifying a rate-limiting step in the pathway\, as well as a set o f catalysts that accelerate the accumulation of the checkpoint effector\, the mitotic checkpoint complex (MCC). Our current efforts aim to unravel t he role of kinetochores in SAC signalling\, using reconstituted material a s our entry point in the investigation. I will report on the conceptual ch allenges associated with this idea\, as well as on our recent experimental progress. LAST-MODIFIED:20191014T130507Z LOCATION:MPI for Medical Research\, Room: Seminar Room A/B ORGANIZER;CN=Prof. Dr. Kai Johnsson:mailto:pr@mpimf-heidelberg.mpg.de SUMMARY:Rudolf Mößbauer Colloquium with Andrea Musacchio (MPI of Molecula r Physiology): Rudolf Mößbauer Colloquium with Andrea Musacchio (MPI of Molecular Physiology) - The kinetochore: the ultimate divisive machine URL;VALUE=URI:https://www.mr.mpg.de/events/19259/10694 END:VEVENT END:VCALENDAR