Evolutionary mutagenesis - optimization of chaperon assisted folding
What key properties of the chaperones have to be changed to achieve defined (selected) functional properties? We modify chaperones by random mutagenesis and select them according to their properties in vivo or in vitro.
Target properties to select for are chaperon assisted survival of cells and improvement of the folding yields of defined target proteins. Specifically, in vitro selection requires the development and use of robot-based automated screening assays.