January 17, 2023
Neuri Christmas
Six-color fluorescence microscopy (5× STED + 1× confocal) in fixed rat hippocampal primary neurons enabled by combining conventional and HCage flurophores developed by Alexey Butkevich. The image shows inhibitory (Gephyrin, red) and excitatory (PSD95, blue) postsynaptic sites with the presynaptic counterpart (Bassoon, yellow) in the context of the membrane associated periodic actin cytoskeleton (beta-II-spectrin, white) and vesicular glutamate transporter 1 (vGLUT1, green). Pseudo volume labelling via actin labelling with phalloidin in cyan. Scale bar is 10 µm. From: Angel Rafael Cereceda Delgado