Publications

Sun, D.-e.; Ng, S. W.; Zheng, Y.; Xie, S.; Schwan, N.; Breuer, P.; Hoffmann, D. C.; Michel, J.; Azorin, D. D.; Boonekamp, K. E. et al.; Winkler, F.; Wick, W.; Boutros, M.; Li, Y.; Johnsson, K.: Molecular recording of cellular protein kinase activity with chemical labeling. Nature Chemical Biology, pp. 1 - 30 (2025)

Kühn, S.; Nasufovic, V.; Wilhelm, J.; Kompa, J.; de Lange, E. M.F.; Lin, Y.-H.; Egoldt, C.; Fischer, J.; Lennoi, A.; Tarnawski, M. et al.; Reinstein, J.; Vlijm, R.; Hiblot, J.; Johnsson, K.: SNAP-tag2: faster and brighter protein labeling. Nature Chemical Biology, pp. 1 - 26 (2025)

Lin, Y.-H.; Kompa, J.; Sun, D.-e.; Mao, R.; Koch, B.; Hinnah, K.; Wilhelm, J.; Franz, N.; Kühn, S.; Menche, T. et al.; Adow, A.; Breuer, P.; Hiblot, J.; Johnsson, K.: A high-affinity split-HaloTag for live-cell protein labeling. bioRxiv, pp. 1 - 52 (2025)

Porzberg, N.; Gries, K.; Johnsson, K.: Exploiting covalent chemical labeling with self-labeling proteins. Annual Review of Biochemistry 94, pp. 29 - 58 (2025)

Bayer, K. V.; Taeb, M.; Koch, B.; Yoshimura, S. H.; Wombacher, R.: Dual SLIPT - a lipid mimic to enable spatiotemporally defined, sequential protein dimerization. ACS Chemical Biology 20 (5), pp. 1038 - 1047 (2025)

Wilhelm, J.; Nickel, L.; Lin, Y.-H.; Hiblot, J.; Johnsson, K.: Improving split-HaloTag through computational protein engineering. Protein Science 34 (5), e70123, pp. 1 - 10 (2025)

Bastl, C.; Close, C. M.; Holtz, I.; Gatin-Fraudet, B.; Eis, M.; Werum, M.; Konrad, S.; Kneller, L.; Roßmann, K.; Huhn, C. et al.; Ghosh, S.; Ast, J.; Roosen, D. A.; Lehmann, M.; Haucke, V.; Reymond, L.; Hodson, D. J.; Tinnefeld, P.; Johnsson, K.; Glembockyte, V.; Kilian, N.; Broichhagen, J.: A silicon rhodamine-fused glibenclamide to label and detect malaria-infected red blood cells. Chembiochem 26 (7), e202400628, pp. 1 - 9 (2025)

Nasufovic, V.; Kompa, J.; Lindamood, H. L.; Blümke, M.; Koch, B.; Le-vario-Diaz, V.; Weber, K.; Maager, M.; Cavalcanti-Adam, E. A.; Vitriol, E. A. et al.; Arndt, H.-D.; Johnsson, K.: SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells. bioRxiv, 636537, pp. 1 - 15 (2025)

Pöschko, P.; Berrou, C. M.; Pakari, K.; Ziegler, M. J.; Kern, C.; Koch, B.; Wittbrodt, J.; Wombacher, R.: Photoactivatable plant hormone-based chemical inducers of proximity for in vivo applications. ACS Chemical Biology 20, pp. 332 - 339 (2025)

Gouveia, G.; Saateh, A.; Swietlikowska, A.; Scarpellini, C.; Tsang, E.; Altug, H.; Merkx, M.; Dillen, A.; Leirs, K.; Spasic, D. et al.; Lammertyn, J.; Gothelf, K. V.; Bonedeau, E.; Porzberg, N.; Smeets, R. L.; Koenen, H. J. P. M.; Prins, M. W. J.; de Jonge, M. I.: Continuous biosensing to monitor acute systemic inflammation, a diagnostic need for therapeutic guidance. ACS Sensors 10 (1), pp. 4 - 14 (2025)

Catapano, C.; Dietz, M. S.; Kompa, J.; Jang, S.; Freund, P.; Johnsson, K.; Heilemann, M.: Long-term single-molecule tracking in living cells using weak-affinity protein labeling. Angewandte Chemie International Edition 64 (1), e202413117, pp. 1 - 8 (2025)

Zheng, Y.; Cai, R.; Wang, K.; Zhang, J.; Zhuo, Y.; Dong, H.; Zhang, Y.; Wang, Y.; Deng, F.; Ji, E. et al.; Cui, Y.; Fang, S.; Zhang, X.; Zhang, K.; Wang, J.; Li, G.; Miao, X.; Wang, Z.; Yang, Y.; Li, S.; Grimm, J.; Johnsson, K.; Schreiter, E.; Lavis, L.; Chen, Z.; Mu, Y.; Li, Y.: In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors. bioRxiv, pp. 1 - 56 (2024)

Liu, T.; Kompa, J.; Ling, J.; Lardon, N.; Zhang, Y.; Chen, J.; Reymond, L.; Chen, P.; Tran, M.; Yang, Z. et al.; Zhang, H.; Liu, Y.; Pitsch, S.; Zou, V. O. P.; Wang, L.; Johnsson, K.; Chen, Z.: Gentle rhodamines for live-cell fluorescence microscopy. ACS Central Science 10 (10), pp. 1933 - 1944 (2024)

Wang, M.-M.; Johnsson, K.: Metal-free introduction of primary sulfonamide into electron-rich aromatics. Chemical Science 15 (31), pp. 12310 - 12315 (2024)

Hellweg, L.; Pfeifer, M.; Tarnawski, M.; Thing-Teoh, S.; Chang, L.; Bergner, A.; Kress, J.; Hiblot, J.; Wiedmer, T.; Superti-Furga, G. et al.; Reinhardt, J.; Johnsson, K.; Leippe, P.: AspSnFR: A genetically encoded biosensor for real-time monitoring of aspartate in live cells. Cell Chemical Biology 31 (8), pp. 1529 - 1541.e12 (2024)

Chang, H.; Clemens, S.; Gao, P.; Li, Q.; Zhao, H.; Wang, L.; Zhang, J.; Zhou, P.; Johnsson, K.; Wang, L.: Fluorogenic rhodamine-based chemigenetic biosensor for monitoring cellular NADPH dynamics. Journal of the American Chemical Society 146 (30), pp. 20569 - 20576 (2024)

Neukam, M.; Sala, P.; Brunner, A.-D.; Ganß, K.; Palladini, A.; Grzybek, M.; Topcheva, O.; Vasiljević, J.; Broichhagen, J.; Johnsson, K. et al.; Kurth, T.; Mann, M.; Coskun, Ü.; Solimena, M.: Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging. Cell Reports 43 (3), 113836, pp. 1 - 23 (2024)

Huppertz, M.-C.; Wilhelm, J.; Grenier, V.; Schneider, M. W.; Falt, T.; Porzberg, N.; Hausmann, D.; Hoffmann, D. C.; Hai, L.; Tarnawski, M. et al.; Pino, G.; Slanchev, K.; Kolb, I.; Acuna, C.; Fenk, L. M.; Baier, H.; Hiblot, J.; Johnsson, K.: Recording physiological history of cells with chemical labeling. Science 383 (6685), pp. 890 - 897 (2024)

Hai, L.; Hoffmann, D. C.; Wagener, R. J.; Azorin, D. D.; Hausmann, D.; Xie, R.; Huppertz, M.-C.; Hiblot, J.; Sievers, P.; Heuer, S. et al.; Ito, J.; Cebulla, G.; Kourtesakis, A.; Kaulen, L. D.; Ratliff, M.; Mandelbaum, H.; Jung, E.; Jabali, A.; Horschitz, S.; Ernst, K. J.; Reibold, D.; Warnken, U.; Venkataramani, V.; Will, R.; Suvà, M. L.; Herold-Mende, C.; Sahm, F.; Winkler, F.; Schlesner, M.; Wick, W.; Kessler, T.: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1. Nature Communications 15 (1), 968, pp. 1 - 29 (2024)

Relimpio, A. Y.; Fink, A.; Bui, D. T.; Fabritz, S.; Schröter, M.; Ruggieri, A.; Platzman, I.; Spatz, J. P.: Bottom-up assembled synthetic SARS-CoV-2 miniviruses reveal lipid membrane affinity of omicron variant spike glycoprotein. ACS Nano, pp. 23913 - 23923 (2023)