Publications of Kai Johnsson

Kühn, S.; Nasufovic, V.; Wilhelm, J.; Kompa, J.; de Lange, E. M.F.; Lin, Y.-H.; Egoldt, C.; Fischer, J.; Lennoi, A.; Tarnawski, M. et al.; Reinstein, J.; Vlijm, R.; Hiblot, J.; Johnsson, K.: SNAP-tag2: faster and brighter protein labeling. Nature Chemical Biology, pp. 1 - 26 (2025)

Lin, Y.-H.; Kompa, J.; Sun, D.-e.; Mao, R.; Koch, B.; Hinnah, K.; Wilhelm, J.; Franz, N.; Kühn, S.; Menche, T. et al.; Adow, A.; Breuer, P.; Hiblot, J.; Johnsson, K.: A high-affinity split-HaloTag for live-cell protein labeling. bioRxiv, pp. 1 - 52 (2025)

Porzberg, N.; Gries, K.; Johnsson, K.: Exploiting covalent chemical labeling with self-labeling proteins. Annual Review of Biochemistry 94, pp. 29 - 58 (2025)

Wilhelm, J.; Nickel, L.; Lin, Y.-H.; Hiblot, J.; Johnsson, K.: Improving split-HaloTag through computational protein engineering. Protein Science 34 (5), e70123, pp. 1 - 10 (2025)

Bastl, C.; Close, C. M.; Holtz, I.; Gatin-Fraudet, B.; Eis, M.; Werum, M.; Konrad, S.; Kneller, L.; Roßmann, K.; Huhn, C. et al.; Ghosh, S.; Ast, J.; Roosen, D. A.; Lehmann, M.; Haucke, V.; Reymond, L.; Hodson, D. J.; Tinnefeld, P.; Johnsson, K.; Glembockyte, V.; Kilian, N.; Broichhagen, J.: A silicon rhodamine-fused glibenclamide to label and detect malaria-infected red blood cells. Chembiochem 26 (7), e202400628, pp. 1 - 9 (2025)

Nasufovic, V.; Kompa, J.; Lindamood, H. L.; Blümke, M.; Koch, B.; Le-vario-Diaz, V.; Weber, K.; Maager, M.; Cavalcanti-Adam, E. A.; Vitriol, E. A. et al.; Arndt, H.-D.; Johnsson, K.: SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells. bioRxiv, 636537, pp. 1 - 15 (2025)

Catapano, C.; Dietz, M. S.; Kompa, J.; Jang, S.; Freund, P.; Johnsson, K.; Heilemann, M.: Long-term single-molecule tracking in living cells using weak-affinity protein labeling. Angewandte Chemie International Edition 64 (1), e202413117, pp. 1 - 8 (2025)

Zheng, Y.; Cai, R.; Wang, K.; Zhang, J.; Zhuo, Y.; Dong, H.; Zhang, Y.; Wang, Y.; Deng, F.; Ji, E. et al.; Cui, Y.; Fang, S.; Zhang, X.; Zhang, K.; Wang, J.; Li, G.; Miao, X.; Wang, Z.; Yang, Y.; Li, S.; Grimm, J.; Johnsson, K.; Schreiter, E.; Lavis, L.; Chen, Z.; Mu, Y.; Li, Y.: In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors. bioRxiv, pp. 1 - 56 (2024)

Liu, T.; Kompa, J.; Ling, J.; Lardon, N.; Zhang, Y.; Chen, J.; Reymond, L.; Chen, P.; Tran, M.; Yang, Z. et al.; Zhang, H.; Liu, Y.; Pitsch, S.; Zou, V. O. P.; Wang, L.; Johnsson, K.; Chen, Z.: Gentle rhodamines for live-cell fluorescence microscopy. ACS Central Science 10 (10), pp. 1933 - 1944 (2024)

Wang, M.-M.; Johnsson, K.: Metal-free introduction of primary sulfonamide into electron-rich aromatics. Chemical Science 15 (31), pp. 12310 - 12315 (2024)

Hellweg, L.; Pfeifer, M.; Tarnawski, M.; Thing-Teoh, S.; Chang, L.; Bergner, A.; Kress, J.; Hiblot, J.; Wiedmer, T.; Superti-Furga, G. et al.; Reinhardt, J.; Johnsson, K.; Leippe, P.: AspSnFR: A genetically encoded biosensor for real-time monitoring of aspartate in live cells. Cell Chemical Biology 31 (8), pp. 1529 - 1541.e12 (2024)

Chang, H.; Clemens, S.; Gao, P.; Li, Q.; Zhao, H.; Wang, L.; Zhang, J.; Zhou, P.; Johnsson, K.; Wang, L.: Fluorogenic rhodamine-based chemigenetic biosensor for monitoring cellular NADPH dynamics. Journal of the American Chemical Society 146 (30), pp. 20569 - 20576 (2024)

Neukam, M.; Sala, P.; Brunner, A.-D.; Ganß, K.; Palladini, A.; Grzybek, M.; Topcheva, O.; Vasiljević, J.; Broichhagen, J.; Johnsson, K. et al.; Kurth, T.; Mann, M.; Coskun, Ü.; Solimena, M.: Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging. Cell Reports 43 (3), 113836, pp. 1 - 23 (2024)

Huppertz, M.-C.; Wilhelm, J.; Grenier, V.; Schneider, M. W.; Falt, T.; Porzberg, N.; Hausmann, D.; Hoffmann, D. C.; Hai, L.; Tarnawski, M. et al.; Pino, G.; Slanchev, K.; Kolb, I.; Acuna, C.; Fenk, L. M.; Baier, H.; Hiblot, J.; Johnsson, K.: Recording physiological history of cells with chemical labeling. Science 383 (6685), pp. 890 - 897 (2024)

Güldenpfennig, A.; Hopp, A.-K.; Muskalla, L.; Manetsch, P.; Raith, F.; Hellweg, L.; Dördelmann, C.; Pedrioli, D. M. L.; Johnsson, K.; Superti-Furga, G. et al.; Hottiger, M. O.: Absence of mitochondrial SLC25A51 enhances PARP1-dependent DNA repair by increasing nuclear NAD+ levels. Nucleic Acids Research 51 (17), pp. 9248 - 9265 (2023)

Hellweg, L.; Edenhofer, A.; Barck, L.; Huppertz, M.-C.; Frei, M. S.; Tarnawski, M.; Bergner, A.; Koch, B.; Johnsson, K.; Hiblot, J.: A general method for the development of multicolor biosensors with large dynamic ranges. Nature Chemical Biology 19 (9), pp. 1147 - 1157 (2023)

Salim, A.; Werther, P.; Hatzopoulos, G. N.; Reymond, L.; Wombacher, R.; Gönczy, P.; Johnsson, K.: Chemical probe for imaging of polo-like Kinase 4 and centrioles. JACS Au 3 (8), pp. 2247 - 2256 (2023)

Jang, S.; Narayanasamy, K. K.; Rahm, J. V.; Saguy, A.; Kompa, J.; Dietz, M. S.; Johnsson, K.; Shechtman, Y.; Heilemann, M.: Neural network-assisted single-molecule localization microscopy with a weak-affinity protein tag. Biophysical Reports 3 (3), 100123, pp. 1 - 9 (2023)

Nanba, D.; Sakabe, J.-I.; Mosig, J.; Brouard, M.; Toki, F.; Shimokawa, M.; Kamiya, M.; Braschler, T.; Azzabi, F.; Lathion, S. D.-G. et al.; Johnsson, K.; Roy, K.; Schmid, C. D.; Bureau, J.-B.; Rochat, A.; Barrandon, Y.: Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures. EMBO Reports 24 (6), e55439, pp. 1 - 19 (2023)

Holtmannspötter, M.; Wienbeuker, E.; Dellmann, T.; Watrinet, I.; Garcia-Sáez, A. J.; Johnsson, K.; Kurre, R.; Piehler, J.: Reversible live-cell labeling with retro-engineered HaloTags enables long-term high- and super-resolution imaging. Angewandte Chemie International Edition 62 (18), e202219050, pp. 1 - 8 (2023)